I remember reading that Twinky Winkie being gay was because the actor playing him was.
For Big Ears, I heard something along the lines of when the books originally came out there were some scenes showed Big Ears and Noddy having a sleepover(?) where they shared a bed.
Have you heard the smurf one? Apparently when the Smurfs first came out they were rumours that they were all gay/it was promoting homosexuality. Then the creators made Smurfette and all the other smurfs are all in love with her.
